https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Endocytosis inhibition in humans to improve responses to ADCC-mediating antibodies https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46366 in vivo may have disruptive therapeutic potential. Here, we demonstrate that the anti-emetic/anti-psychotic prochlorperazine can be repurposed to reversibly inhibit the in vivo endocytosis of membrane proteins targeted by therapeutic monoclonal antibodies, as directly demonstrated by our human tumor ex vivo assay. Temporary endocytosis inhibition results in enhanced target availability and improved efficiency of natural killer cell-mediated antibody-dependent cellular cytotoxicity (ADCC), a mediator of clinical responses induced by IgG1 antibodies, demonstrated here for cetuximab, trastuzumab, and avelumab. Extensive analysis of downstream signaling pathways ruled out on-target toxicities. By overcoming the heterogeneity of drug target availability that frequently characterizes poorly responsive or resistant tumors, clinical application of reversible endocytosis inhibition may considerably improve the clinical benefit of ADCC-mediating therapeutic antibodies.]]> Wed 16 Nov 2022 08:57:15 AEDT ]]> Dynamin regulates L cell secretion in human gut https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40290 Thu 07 Jul 2022 14:57:42 AEST ]]> Green chemistry approaches to the Knoevenagel condensation: comparison of ethanol, water and solvent free (dry grind) approaches https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:3268 Sat 24 Mar 2018 07:21:22 AEDT ]]> Safety of bronchoalveolar lavage in young children with cystic fibrosis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4767 Sat 24 Mar 2018 07:20:41 AEDT ]]>